Immunodesign of experimental sepsis by cecal ligation and puncture
نویسندگان
چکیده
منابع مشابه
Cecal ligation and puncture induced sepsis
Purpose: Multiresistant and vancomycin resistant Enterococcus faecium (VRE) can cause serious infections in hospitalized patients with various co-morbid diseases. We investigated the course of VRE peritonitis after cecal ligation and puncture (CLP)-induced sepsis and compared this to sham operated mice. Methods: Mice were subjected to CLP or sham surgery. Forty-eight hours thereafter four group...
متن کاملA New Approach to Monitoring and Evaluation of Cecal Ligation and Puncture Sepsis Model
Background: Sepsis is a systemic inflammatory disease in response to the pathogens that leads to vital organ failures the failure of vital organs. Appropriate animal models should be developed to measure the effectiveness of therapeutic methods. Cecal Ligation and Puncture (CLP) is the most widely used methods of creating the sepsis model. Some variables interfere in the creation of the CLP mod...
متن کاملCecal ligation puncture procedure.
Human sepsis is characterized by a set of systemic reactions in response to intensive and massive infection that failed to be locally contained by the host. Currently, sepsis ranks among the top ten causes of mortality in the USA intensive care units. During sepsis there are two established haemodynamic phases that may overlap. The initial phase (hyperdynamic) is defined as a massive production...
متن کاملProfiling Circulating MicroRNA Expression in Experimental Sepsis Using Cecal Ligation and Puncture
The levels of circulating microRNAs (miRNAs) in mice with experimental sepsis induced by cecal ligation and puncture (CLP) were determined using whole blood samples obtained from C57BL/6 mice at 4, 8, and 24 h after CLP; miRNA expression analysis was performed in these samples using an miRNA array. Microarray analysis revealed upregulation of 10 miRNA targets (miR-16, miR-17, miR-20a, miR-20b, ...
متن کاملValidation of a Murinic Model of Sepsis Induced by Cecal Ligation and Puncture Technique
Sepsis is considered to be a systemic response to an infection. This pathology is an common cause for mortality and morbidity being the primary cause of death among patients in the Intensive Care Units. Because of the increasing need for a standardised animal model of sepsis we decided to validate a murinic one using the cecal ligation and puncture (CLP) technique and easy to obtain physiologic...
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ژورنال
عنوان ژورنال: Nature Protocols
سال: 2008
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2008.214